The perceived potential of religion in mitigating climate change and how this is being realized in Germany and Switzerland.

Scholars of religion have repeatedly debated and contested the role of religion and spirituality in combatting climate change. In recent years, the potential of religion has also become an issue among natural scientists, politicians, environmental organizations, and civil society. Indeed, the potential of religion to mitigate climate change is perceived both internally and externally, and various expectations are placed on religion. This article examines the perceived potential of religion in mitigating climate change and how this is being realized. Based on 38 interviews, conducted with representatives from religious communities and umbrella organizations in Germany and Switzerland, we focus on the areas of values, political influence, and materialization. Our results show that the potential of religion in addressing climate change remains largely unfulfilled despite increasing steps in this direction.

Antibody-dye Conjugates Targeting EGFR and HER2 for the Photoimmunotherapy of Bladder Cancer.

BACKGROUND/AIM: Although there are curative treatment options for non-muscle-invasive bladder cancer, the recurrence of this tumor is high. Therefore, novel targeted therapies are needed for the complete removal of bladder cancer cells in stages of localized disease, in order to avoid local recurrence, to spare bladder cancer patients from stressful and expensive treatment procedures and to increase their quality of life and life expectancy. This study tested a new approach for the photoimmunotherapy (PIT) of bladder cancer. MATERIALS AND METHODS: We generated a cysteine modified recombinant version of the antibody cetuximab targeting the epidermal growth factor receptor (EGFR) on the surface of bladder cancer cells. Then, we coupled the novel photoactivatable phthalocyanine dye WB692-CB1 via a maleimide linker to the free cysteines of the antibody. PIT was performed by incubating bladder cancer cells with the antibody dye conjugate followed by irradiation with visible red light. RESULTS: The conjugate was able to induce specific cytotoxicity in EGFR-positive bladder cancer cells in a light dose-dependent manner. Enhanced cytotoxicity in RT112 bladder cancer cells was evoked by addition of a second antibody dye conjugate targeting HER2 or by repeated cycles of PIT. CONCLUSION: Our new antibody dye conjugate targeting EGFR-expressing bladder cancer cells is a promising candidate for the future PIT of bladder cancer patients.

How "green" can religions be? Tensions about religious environmentalism.

Scholarship has suggested a "greening" of religions, supposing that faith communities increasingly become environmentally friendly and use their potentials to address environmental challenges. This contribution points to the problems of the supposed "greening" by indicating the ongoing disagreements in many religious traditions over environmental engagement. The disagreements show that religious environmentalism is an embattled terrain that involves actors with different interests, backgrounds, and understandings of their traditions. The authors illustrate that tensions are an inherent part of religious environmentalism, becoming manifest in different views and theologies, ambivalences, misunderstandings, and sometimes mistrust. They distinguish between four types of tensions: (1) intradenominational tensions, (2) interdenominational tensions, (3) interreligious tensions, and (4) religious-societal tensions. By drawing attention to the tensions of religious environmentalism, this contribution sheds light on the struggles and limitations that religious environmentalists face in their ambitions to address climate change and other environmental challenges.

Boosting Efficiency in Light-Driven Water Splitting by Dynamic Irradiation through Synchronizing Reaction and Transport Processes.

This work elaborates the effect of dynamic irradiation on light-driven molecular water oxidation to counteract deactivation. It highlights the importance of overall reaction engineering to overcome limiting factors in artificial photosynthesis reactions. Systematic investigation of a homogeneous three-component ruthenium-based water oxidation system revealed significant potential to enhance the overall catalytic efficiency by synchronizing the timescales of photoreaction and mass transport in a capillary flow reactor. The overall activity could be improved by a factor of more than 10 with respect to the turnover number and a factor of 31 referring to the external energy efficiency by controlling the local availability of photons. Detailed insights into the mechanism of light driven water oxidation could be obtained using complementary methods of investigation like Raman, IR, and UV/Vis/emission spectroscopy, unraveling the importance of avoiding high concentrations of excited photosensitizers.

Influence of 3D Printing Parameters on the Mechanical Stability of PCL Scaffolds and the Proliferation Behavior of Bone Cells.

Introduction The use of scaffolds in tissue engineering is becoming increasingly important as solutions need to be found for the problem of preserving human tissue, such as bone or cartilage. In this work, scaffolds were printed from the biomaterial known as polycaprolactone (PCL) on a 3D Bioplotter. Both the external and internal geometry were varied to investigate their influence on mechanical stability and biocompatibility. Materials and Methods: An Envisiontec 3D Bioplotter was used to fabricate the scaffolds. First, square scaffolds were printed with variations in the strand width and strand spacing. Then, the filling structure was varied: either lines, waves, and honeycombs were used. This was followed by variation in the outer shape, produced as either a square, hexagon, octagon, or circle. Finally, the internal and external geometry was varied. To improve interaction with the cells, the printed PCL scaffolds were coated with type-I collagen. MG-63 cells were then cultured on the scaffolds and various tests were performed to investigate the biocompatibility of the scaffolds. Results: With increasing strand thickness and strand spacing, the compressive strengths decreased from 86.18 + 2.34 MPa (200 µm) to 46.38 + 0.52 MPa (600 µm). The circle was the outer shape with the highest compressive strength of 76.07 + 1.49 MPa, compared to the octagon, which had the lowest value of 52.96 ± 0.98 MPa. Varying the external shape (toward roundness) geometry, as well as the filling configuration, resulted in the highest values of compressive strength for the round specimens with honeycomb filling, which had a value of 91.4 + 1.4 MPa. In the biocompatibility tests, the round specimens with honeycomb filling also showed the highest cell count per mm2, with 1591 ± 239 live cells/mm2 after 10 days and the highest value in cell proliferation, but with minimal cytotoxic effects (9.19 ± 2.47% after 3 days).

Evaluation of Ki-67 as a Prognostic Marker in Diffuse Large B-Cell Lymphoma-A Single-Center Retrospective Cohort Study.

BACKGROUND: Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma and prognostic information is essential in finding the right treatment. This study evaluated the prognostic significance of Ki-67 in patients with DLBCL. METHODS: Patients with DLBCL, treated with first-line R-CHOP, were retrospectively analyzed in groups of high (>70%) and low (≤70%) Ki-67. Parameters of interest were the international prognostic index (IPI), treatment response, progression-free survival (PFS) and overall survival (OS). A chi-squared test or Fisher's exact test was conducted to analyze categorical variables. Kaplan-Meier and log-rank tests were applied for survival analyses. Finally, a multivariate linear regression analysis was performed, including gender, Ki-67 ≤ 70% or >70%, IPI and presence of B symptoms. RESULTS: Overall, 58 patients were included. No significant association was found between Ki-67 status and IPI (p = 0.148) or treatment response (p = 0.373). Survival in patients with high Ki-67 was significantly inferior with respect to OS (p = 0.047) but not PFS (p = 0.138). Multivariate linear regression, however, yielded only IPI as a risk factor for OS. CONCLUSION: Future studies with larger patient cohorts are needed in order to elucidate the prognostic role of Ki-67 in patients with DLBCL treated with R-CHOP.

Switching the Mechanism of NADH Photooxidation by Supramolecular Interactions.

A series of three Ru(II) polypyridine complexes was investigated for the selective photocatalytic oxidation of NAD(P)H to NAD(P)+ in water. A combination of (time-resolved) spectroscopic studies and photocatalysis experiments revealed that ligand design can be used to control the mechanism of the photooxidation: For prototypical Ru(II) complexes a 1 O2 pathway was found. Rudppz ([(tbbpy)2 Ru(dppz)]Cl2 , tbbpy=4,4'-di-tert-butyl-2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c]phenazine), instead, initiated the cofactor oxidation by electron transfer from NAD(P)H enabled by supramolecular binding between substrate and catalyst. Expulsion of the photoproduct NAD(P)+ from the supramolecular binding site in Rudppz allowed very efficient turnover. Therefore, Rudppz permits repetitive selective assembly and oxidative conversion of reduced naturally occurring nicotinamides by recognizing the redox state of the cofactor under formation of H2 O2 as additional product. This photocatalytic process can fuel discontinuous photobiocatalysis.

A Ruthenium(II) Water Oxidation Catalyst Containing a pH-Responsive Ligand Framework.

The synthesis of a new RuII-based water oxidation catalyst is presented, in which a nitrophenyl group is introduced into the backbone of dpp via a pH-sensitive imidazole bridge (dpp = 2,9-di-(2'-pyridyl)-1,10-phenanthroline). This modification had a pronounced effect on the photophysical properties and led to the appearance of a significant absorption band around 441 nm in the UV-vis spectrum upon formation of the monoprotonated species under neutral conditions. Theoretical investigations could show that the main contributions to this band arise from transitions involving the imidazole and nitrophenyl motif, allowing us to determine the pKa value (6.8 ± 0.1) of the corresponding, twofold protonated conjugated acid. In contrast, the influence of the nitrophenyl group on the electrochemical properties of the catalytic center was negligible. Likewise, the catalytic performance of Ru(dppip-NO2) and its parent complex Ru(dpp) was comparable over the entire investigated pH range (dppip-NO2 = 2-(4-nitrophenyl)-6,9-di(pyridin-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline). This allowed the original catalytic properties to be retained while additionally featuring a functionalized ligand scaffold, which provides further modification opportunities as well as the ability to report the pH of the catalytic solution via UV-vis spectroscopy.

FOXA1 promotes tumor progression in prostate cancer and represents a novel hallmark of castration-resistant prostate cancer.

Forkhead box protein A1 (FOXA1) modulates the transactivation of steroid hormone receptors and thus may influence tumor growth and hormone responsiveness in prostate cancer. We therefore investigated the correlation of FOXA1 expression with clinical parameters, prostate-specific antigen (PSA) relapse-free survival, and hormone receptor expression in a large cohort of prostate cancer patients at different disease stages. FOXA1 expression did not differ significantly between benign glands from the peripheral zone and primary peripheral zone prostate carcinomas. However, FOXA1 was overexpressed in metastases and particularly in castration-resistant cases, but was expressed at lower levels in both normal and neoplastic transitional zone tissues. FOXA1 levels correlated with higher pT stages and Gleason scores, as well as with androgen (AR) and estrogen receptor expression. Moreover, FOXA1 overexpression was associated with faster biochemical disease progression, which was pronounced in patients with low AR levels. Finally, siRNA-based knockdown of FOXA1 induced decreased cell proliferation and migration. Moreover, in vitro tumorigenicity was inducible by ARs only in the presence of FOXA1, substantiating a functional cooperation between FOXA1 and AR. In conclusion, FOXA1 expression is associated with tumor progression, dedifferentiation of prostate cancer cells, and poorer prognosis, as well as with cellular proliferation and migration and with AR signaling. These findings suggest FOXA1 overexpression as a novel mechanism inducing castration resistance in prostate cancer.